Coding
DnaK

Part:BBa_K205002:Design

Designed by: Michael James George Milevskiy   Group: iGEM09_UQ-Australia   (2009-09-28)

DnaK is a chaperone protein that assists with polypeptide folding


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 1671
    Illegal AgeI site found at 399
    Illegal AgeI site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1588


Design Notes

In order to comply with Standard 23, two restriction sites were removed within the gene. These sites were EcoRI and BglII, which were removed through mutagenesis.


Source

Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

References

DnaK: http://www.uniprot.org/uniprot/P0A6Y8